Laboratory manual-urine bench manual(processing of urine in the lab)

we are supposed to make a laboratory manual for a country side laboratory. we need to give the lab a name and water mark.
there needs to be a content and an amendment record.
every page needs to be referenced at the bottom in VANCOUVER style,but no numbers need to be mentioned in text.
every new section should begin from a new page.
MARKING RUBRIC SHOULD BE STRICTLY FOLLOWED.
i have already sent a few details, i will be sending a few documents aswell.

• This will be a laboratory manual in order to process urine sample in our own country side microbiology laboratory.
• It should have headings like  title,purpose,scope,responsibility,clinical significance,reagents,test principle, sample requirements, materials, procedure(divide sub sections when needed , recording data , calculations(if any), quality control, reports(positive or negative), references and authorisation of each test. Authorisation will be like

URINE MANUAL	AUTHORISED	VERSION 1	ISSUE DATE

• References will be added on the end of each test (it can be package insert) or any other (at least 1 or 2) same for introduction.
• Every section should tart from new page as well as headings and subheadings should be clear.
• Use of précised words or dot points if possible especially for procedure.
• Strictly follow the marking rubric (I have mailed you).
• Page 1 should have laboratory name as well as logo of lab (it can be any) and pictures too can be microscopy or culture of urine.
• Page 2 will be content table.(from rubric)
• Page 3 will be Amendment record.
• Page 4 will be introduction about manual and some of UTI causes and organisms involved.(guidelines in rubric)
• Page 5 will be reception of sample in lab (labelling , rejections etc for urine sample in marking rubric)
• Page 6 is processing protocol (how to process and record the results , it can be worksheet or directly to computer – short and précised mentioned in rubric)
• Page 7 is Manual dipstick testing (it can be Roche chemistrip 10 MD or any other) and also mention headings (mentions above) especially QC.
• Then is cell count (using plastic chamber of FAST READ) and microscopy (using phase contrast microscope) each should be mentioned. Urine must not be centrifuged for any test. Directly take the urine for cell counting and microscopy.(must show calculations for counting chamber and normal abnormal count in /L)
• Then is culture processing Medias using calibrated loop on HBA and Chromogenic media on a split plate. Add a table showing colony colour and texture like (pictures can be added) for some UTI organisms.Coloy count calculations. Reading culture ( from rubric)

colour

Presumptive organism

Biochemical test

Results

 

• Microorganisms identification using spot test especially mention catalase test, coagulase test , oxidase test and indole test with procedures ( and all the other points from marking rubric).Their positive and negative results too along with QC .
• Sensitivity method will be CLSI METHOD along with QC.(MARKING RUBRIC)
• Then will be disposal / storage policy (in rubric)
• Referral policy (in rubric)
• Medicare/ NATA/ ISO 17025 should be mentioned on last page (IN RUBRIC).

The scenario for the project in medical microbiology is as follows.

 

You are working in a country hospital that has focused on specimens of an ‘urgent’ nature. The pathology department has a haematology analyser, a biochemistry analyser and an immunology instrument. In microbiology, the scope has been limited to analysis for urgent or life-threatening cases and so a blood culture instrument is available. The department cultures and performs microscopy of CSFs (cerebrospinal fluids). It also does ‘urgent’ Gram stains and urine microscopy. All other specimens are sent to a pathology laboratory in Melbourne by courier.

 

The hospital services a wide rural area and the township where the hospital is located has itself grown greatly in population. The pathology department is sending more and more specimens to the city for processing each day. This results in delays in turnaround time, sometimes misplaced specimens and much time spent preparing the paperwork to accompany specimens. The cost often exceeds the income generated.

 

The hospital has decided to expand its microbiology services to overcome these problems. By far the most common specimen received by a diagnostic microbiology department is a urine specimen. Urines comprise 55-60% of all specimens received by microbiology. It has therefore been decided to start a ‘Urine bench’ and process all urine specimens in-house.

 

You are a recently qualified Medical Laboratory Scientist from RMIT and you have been hired to undertake this project.

Report and oral presentation on assigned method (20%)(16 August 2012)

You must research the method assigned to you to analyse urine specimens. Each student will research one method in detail and will report back to the rest of the class with a 5-minute oral presentation (10%) and a 2-page dot-point presentation of the test, its advantages and disadvantages, cost, availability and final evaluation, together with the most useful of your references (10%).

 

The headings to be used for the dot points are:

• a description of the medium/test/stain/method/instrument
• a short description of the type of infection this test is used to diagnose
• how used
• principle of the test
• cost
• advantages and disadvantages
• ease of use
• turnaround time
• your evaluation of whether or not it should be included in the manual (other students will make their own judgement of each test during the writing of the review. Not all students will come to the same conclusion. This is fine so long as it is justified.)

 

Review and justification of proposed methods (10%)(2000-2500 words)*

From your report and that of your peers (your fellow students), you will review the methods available, select which are the most appropriate and justify your selection fully in terms of value (both quality and cost). You are expected to research the literature thoroughly and critically evaluate the options available.

 

When this area is added to your microbiology department, it will need to be NATA accredited and so any methodology you select must be adequate for the task and meet the standards and guidelines set out by NPAAC (the National Pathology Accreditation Advisory Committee). The results of NATA audits are considered by Medicare Australia in determining access to the Medicare Benefits Scheme, which is how your laboratory is paid for its work.

 

The Medicare schedule is quite specific about what is required for each item. Urine analysis is item 69333 in the Medicare schedule and is described as follows:

‘Urine examination by any means other than simple culture by dip slide, including:

• cell count and
• culture and
• colony count and
• (if performed) stained preparations and
• (if performed) identification of cultured pathogens and
• (if performed) antibiotic susceptibility testing and
• (if performed) examination for pH, specific gravity, blood, albumin, urobilinogen, sugar, acetone or bile salts.’

and so your review of methods should include a method for each of these components.

 

The review should follow the format outlined below:

• Title page
• Table of Contents with pagination
• This should give a brief background to the project, ending with the aims. The background could include something about the hospital and why the review is needed.
• Main content. This will cover (in subsections) your evaluation of the methods available for evaluation of urine specimens in a microbiology pathology laboratory. This will be based on the microbiology literature in which diagnostic methods are evaluated with a head start from the presentations and dot points from other students after the oral presentation. Each test should be evaluated and either accepted or rejected with reasons given, as a test to be included in your laboratory manual. Your evaluation should be based on cost, ease of performance, turnaround time, efficiency and other items that you may think important. This could be arranged in a table by type of test, type of microorganism or some other logical method.
• A short section on which tests you plan to include in your laboratory manual and why.
• Reference list. The review should list references according to the Vancouver method. At least 25 references should be used for this report.
• Submit your review to Turnitin to check for academic integrity. All students will be presenting similar material but the report you submit must be your own work in your own words. You will receive zero marks for this review if any one section is the same as that of another student or published material.

 

After you have submitted your review, it will be marked and you will receive feedback on items ‘missing’ or ‘incorrect’ that would prevent you from obtaining NATA accreditation to ISO/IEC 15189 standard.

Final project report (laboratory manual)(7000 words -30-40 pages)*

You will use these comments and your review information to write a laboratory manual for the new Urine bench. The manual must meet the ISO/IEC15189 guidelines for laboratory manuals. Ask Helen Williams to show you an example of a laboratory manual.

 

Your manual must include:

• Title page
• An Introduction stating the purpose of the manual and other sections required by ISO/IEC 15189
• Table of Contents with pagination
• A method of processing urine specimens from when they arrive in the laboratory until the report is sent to the clinician and the specimens and their tests are disposed of correctly
• A dipstick/chemical analysis method
• A quantitative culture method (standard loop/dipslide/pipetted amount and medium)
• A method for detecting antimicrobial substances
• A method for performing cell count
• Methods for reading and interpretation of cultures
• A method for identification of common microbes isolated from urine
• An antibiotic sensitivity method
• Reference list. The review should list references according to the Vancouver method. Each method must be referenced.
• Submit your review to Turnitin to check for academic integrity. All students will be presenting similar material but the report you submit must be your own work in your own words. You will receive zero marks for this review if any one section is the same as that of another student or published material.

 

Is this the question you were looking for? If so, place your order here to get started!